False-negative latex-agglutination test for myoglobin owing to antigen excess.

CLINICAL CHEMISTRY, Vol. 34, No. 1, 1988 177 the modified Jendrassik-Gr#{243}f procedure in the Technicon SMAC H (x) and with the Dri-STAT reagent in the RA-1000 (y). The resulting linear-regression equation was: y = 0.98x + 0.66 (r = 0.998), range = 4-370 /Lmol/L. The standard curve for total bilirubin was linear within the range 4-370 p.mol/L. To estimate the effect of hemolysis, we prepared four serum poolswith different amounts of bilirubin. Hemoglobin prepared from lysed erythrocytes was added to each pool to give Hb concentrations of 0.5, 1.5, and 2.5 g/L in pooled serum. Results were as follows: